分类： 光学 >> 量子光学 提交时间： 2023-02-19
Shaowei Jiang
Chengfei Guo
Pengming Song
Niyun Zhou
Zichao Bian
Jiakai Zhu
Ruihai Wang
Pei Dong
Zibang Zhang
Jun Liao
Jianhua Yao
Bin Feng
Michael Murphy
Guoan Zheng
摘要： Ptychography is an enabling coherent diffraction imaging technique for both fundamental and applied sciences. Its applications in optical microscopy, however, fall short for its low imaging throughput and limited resolution. Here, we report a resolution-enhanced parallel coded ptychography technique achieving the highest numerical aperture and an imaging throughput orders of magnitude greater than previous demonstrations. In this platform, we translate the samples across the disorder-engineered surfaces for lensless diffraction data acquisition. The engineered surface consists of chemically etched micron-level phase scatters and printed sub-wavelength intensity absorbers. It is designed to unlock an optical space with spatial extent (x, y) and frequency content (kx, ky) that is inaccessible using conventional lens-based optics. To achieve the best resolution performance, we also report a new coherent diffraction imaging model by considering both the spatial and angular responses of the pixel readouts. Our low-cost prototype can directly resolve 308-nm linewidth on the resolution target without aperture synthesizing. Gigapixel high-resolution microscopic images with a 240-mm^2 effective field of view can be acquired in 15 seconds. For demonstrations, we recover slow-varying 3D phase objects with many 2{\pi} wraps, including optical prism and convex lens. The low-frequency phase contents of these objects are challenging to obtain using other existing lensless techniques. For digital pathology applications, we perform accurate virtual staining by using the recovered phase as attention guidance in a deep neural network. Parallel optical processing using the reported technique enables novel optical instruments with inherent quantitative nature and metrological versatility.
分类： 光学 >> 量子光学 提交时间： 2023-02-19
Shaowei Jiang
Chengfei Guo
Zichao Bian
Ruihai Wang
Jiakai Zhu
Pengming Song
Patrick Hu
Derek Hu
Zibang Zhang
Kazunori Hoshino
Bin Feng
Guoan Zheng
摘要： Traditional microbial detection methods often rely on the overall property of microbial cultures and cannot resolve individual growth event at high spatiotemporal resolution. As a result, they require bacteria to grow to confluence and then interpret the results. Here, we demonstrate the application of an integrated ptychographic sensor for lensless cytometric analysis of microbial cultures over a large scale and with high spatiotemporal resolution. The reported device can be placed within a regular incubator or used as a standalone incubating unit for long-term microbial monitoring. For longitudinal study where massive data are acquired at sequential time points, we report a new temporal-similarity constraint to increase the temporal resolution of ptychographic reconstruction by 7-fold. With this strategy, the reported device achieves a centimeter-scale field of view, a half-pitch spatial resolution of 488 nm, and a temporal resolution of 15-second intervals. For the first time, we report the direct observation of bacterial growth in a 15-second interval by tracking the phase wraps of the recovered images, with high phase sensitivity like that in interferometric measurements. We also characterize cell growth via longitudinal dry mass measurement and perform rapid bacterial detection at low concentrations. For drug-screening application, we demonstrate proof-of-concept antibiotic susceptibility testing and perform single-cell analysis of antibiotic-induced filamentation. The combination of high phase sensitivity, high spatiotemporal resolution, and large field of view is unique among existing microscopy techniques. As a quantitative and miniaturized platform, it can improve studies with microorganisms and other biospecimens at resource-limited settings.
分类： 光学 >> 量子光学 提交时间： 2023-02-19
摘要： Fourier single-pixel imaging (FSI) is a branch of single-pixel imaging techniques. It uses Fourier basis patterns as structured patterns for spatial information acquisition in the Fourier domain. However, the spatial resolution of the image reconstructed by FSI mainly depends on the number of Fourier coefficients sampled. The reconstruction of a high-resolution image typically requires a number of Fourier coefficients to be sampled, and therefore takes a long data acquisition time. Here we propose a new sampling strategy for FSI. It allows FSI to reconstruct a clear and sharp image with a reduced number of measurements. The core of the proposed sampling strategy is to perform a variable density sampling in the Fourier space and, more importantly, the density with respect to the importance of Fourier coefficients is subject to a one-dimensional Gaussian function. Combined with compressive sensing, the proposed sampling strategy enables better reconstruction quality than conventional sampling strategies, especially when the sampling ratio is low. We experimentally demonstrate compressive FSI combined with the proposed sampling strategy is able to reconstruct a sharp and clear image of 256-by-256 pixels with a sampling ratio of 10%. The proposed method enables fast single-pixel imaging and provides a new approach for efficient spatial information acquisition.
分类： 光学 >> 量子光学 提交时间： 2023-02-19
摘要： Single-pixel imaging (SPI) is a novel optical imaging technique by replacing the pixelated sensor array in a conventional camera with a single-pixel detector. In previous works, SPI is usually used for capturing object images or performing image processing tasks. In this work, we propose a SPI scheme for processing other types of data in addition to images. An Ising machine model is implemented optically with SPI for solving combinational optimization problems including number partition and graph maximum cut. Simulated and experimental results show that our proposed scheme can optimize the Hamiltonian function with evolutionary illumination patterns.
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